Detection and mapping of DNA methylation with 2D material nanopores
March 25, 2021
Department of Physics
Nano Literature Seminar
Virginia Commonwealth University
Thursday, March 25 at 11:00 am via Zoom
Next-generation high-throughput sequencing has allowed for the generation of genetic data on a large scale that is necessary for determining genetic impacts on human health. With the successful complete sequencing of the human genome, interest has moved towards RNA, protein, and DNA modification sequencing to analyze the transcriptome, proteome, and epigenome respectively. Each of these will require high-resolution and high-throughput sequencing techniques.
In this talk I will discuss the use of 2D materials, particularly graphene and MoS2, in nanopore sequencing devices for mapping DNA methylation sites. DNA methylation is an epigenetic modification that affects many aspects of health and diseases, most notably cancer. Nanopore devices have been used commercially for DNA and RNA sequencing and are promising for the sequencing and mapping of other biomolecules such as methyl-binding domain. It is proposed that by combining ionic current blockade simultaneously with transverse sheet current simulations it is possible to increase mapping resolution while maintaining fast reads.
Matthew Beltran is a Ph.D. student in nanoscience at the Virginia Commonwealth University.